FastQCFastQC Report
Sun 19 Mar 2023
SRR4895712_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895712_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences887896
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA88881.0010181372593188No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG50130.5645931505491635No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG27050.3046527971744439No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC10020.1128510546280195No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA119250.0107.3634958
GTGGTAT119900.0106.5396046
AAGCAGT122150.0105.54461
GTATCAA122500.0104.686739
TGGTATC122200.0104.475027
CAGTGGT124150.0103.0150454
AGCAGTG124700.0102.909542
AGTGGTA125650.0101.785265
GCAGTGG130300.098.1528553
AACCGAG2350.061.710611
ACCGAGA2550.054.020952
CGGTCTG1850.050.94721145
AGCGCAT1153.1613547E-431.522522145
TACGCAC1650.024.6050150-54
GAGGTAT2401.5981823E-624.1659076
CATGGGG40850.022.93261125-29
AGGTATC2552.7072547E-622.7443837
CAACGCA121200.022.3821110-14
AGAGTAC121050.022.37391315-19
CGACCTA1750.022.36946545-49
Produced by FastQC (version 0.11.5)