FastQCFastQC Report
Sun 19 Mar 2023
SRR4895711_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895711_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences744489
Sequences flagged as poor quality0
Sequence length151
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT551897.413004087367309No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGT124721.675243019037219No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT74370.9989402126827931No Hit
AACAACCATGTACTCTGCGTGGATACCACTGCTTCCCATGTACTCTGCGT16720.22458357343090363No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCGCACGGACGCGCCGC15610.20967401801772761No Hit
AACAACCAATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG15210.20430120525622275No Hit
AACAACCATGTACTCTGCGTTGAGACCACTGCTTCCCATGTACTCTGCGT14860.19959999408990597No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGC14800.19879407217568024No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTC11790.15836365614535608No Hit
AACAACCATGTACTCCGCGTTGATACCACTGCTTCCCATGTACTCTGCGT10690.1435884210512177No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTAGAACGGAAGAGCGGC10550.14170793658469097No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCGCACGGACGCGCGGC9960.1337830377614713No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTCCCCATGTACTCTGCGT9330.1253208576621011No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCGTCCCATGTACTCTGCGT8560.11497819309620423No Hit
AACAACCATGTACTCTGCGTTGATACGACTGCTTCCCATGTACTCTGCGT8530.11457523213909138No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCAGGAACCCGCCGT8480.11390363054390328No Hit
AACAACCATGTACCCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT8290.11135154448218844No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTACCCATGTACTCTGCGT7760.1042325675731945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCATG227900.0143.394174
CAACCAT359250.0143.144443
ACAACCA754450.0142.336672
CAACCAG205650.0142.284643
ACCATGT178800.0141.909045
AACAACC759350.0141.418181
AACCAGT71600.0141.02524
AACCATA64450.0138.653264
ACCATAG22350.0138.439975
ACCATTG25750.0137.806475
ACCAGTA20900.0136.656625
ACCATGA20700.0136.583275
AACCAGG76250.0136.208564
ACCAGTG25150.0135.932115
ACCAGTT23750.0135.745575
AACCAAT66850.0135.617454
CAACCAA184600.0135.574973
ACCAGGT26400.0134.95995
ACCAGAT19650.0134.705475
ACCAATG25650.0134.68835
Produced by FastQC (version 0.11.5)