FastQCFastQC Report
Sun 19 Mar 2023
SRR4895710_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895710_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492203
Sequences flagged as poor quality0
Sequence length151
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTACAAGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG5040.10239677531425043No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAG493100.0143.924872
AGTACAA494600.0143.488371
TACAAGT161800.0143.07413
TACAAGG166550.0142.066973
TACAAGA150250.0141.836883
TACAAGC20200.0140.975923
ACAAGTG57900.0140.826144
ACAAGTA50650.0140.62964
CAAGTAG16500.0139.81875
ACAAGAT52150.0139.625994
ACAAGTT54750.0139.579284
CAAGTGA16550.0139.396295
CAAGTTG18700.0139.176155
CAAGATG18650.0139.162725
ACAAGGT58600.0139.143924
CAAGTAT16200.0138.847735
CAAGTGT18850.0138.068655
ACAAGAG52900.0137.918984
CAAGAGT15600.0137.718055
CAAGTTA17950.0136.556925
Produced by FastQC (version 0.11.5)