FastQCFastQC Report
Sun 19 Mar 2023
SRR4895710_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895710_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492203
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG102462.0816614283131147No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44390.9018636619443604No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA15060.3059713167128197No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA113050.077.665315
GGTATCA114500.077.125038
GTGGTAT114000.077.01816
AAGCAGT114500.076.871741
CAGTGGT114700.076.611284
GTATCAA116500.075.979979
AGCAGTG115800.075.946152
GCAGTGG116250.075.5274353
TGGTATC116450.075.522237
CGGTCTG1703.8198777E-1142.64425145
CGAGCCG1050.00770718727.617228145
TACGCAC1550.025.25896850-54
CGACCTA1500.025.13269845-49
CGCAAGG3501.2914825E-1024.855507145
CGCTGTT2101.5537771E-524.165075145
CATGGGG22450.023.25108325-29
CGGAGCC3203.3791366E-822.654757145
GCCCCAA5650.021.812723145
TCGGTAA604.5589513E-721.75077690-94
TCCGGCG1650.021.09166335-39
Produced by FastQC (version 0.11.5)