FastQCFastQC Report
Sun 19 Mar 2023
SRR4895709_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895709_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences657520
Sequences flagged as poor quality0
Sequence length151
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGAT657900.0144.10832
CGTGATA162950.0144.0193
AACGTGA658350.0144.00981
CGTGATG289600.0143.847433
CGTGATT183050.0143.605163
GTGATGA74750.0141.10954
GTGATGG81200.0140.99954
GTGATGT135950.0140.854924
CGTGATC24150.0140.613453
GTGATAG40250.0137.747454
GTGATAT77400.0137.396034
GTGATTA51700.0137.223244
GTGATAA51450.0137.049224
TGATGTG57750.0135.831365
GTGATTG47050.0135.767944
GTGATCG6200.0134.8934
TGATGAG28450.0134.819435
TGATGTA33550.0131.087255
TGATAAG21900.0130.368965
TGATATG33500.0130.206825
Produced by FastQC (version 0.11.5)