FastQCFastQC Report
Sun 19 Mar 2023
SRR4895709_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895709_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences657520
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG164862.5073001581700938No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA57610.876171067039786No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA26280.39968365981262927No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT7210.1096544591799489No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC7010.10661272660907653No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT185500.079.160561
AGTGGTA184850.078.799275
GGTATCA188200.077.544828
GTGGTAT187850.077.496346
AGCAGTG189700.077.140352
GTATCAA189550.077.1072859
CAGTGGT190000.076.751394
TGGTATC190500.076.380257
GCAGTGG193900.075.095463
CGTGATT1252.7126407E-740.608393
AACGTGA3400.036.2574961
ACGTGAT3850.033.903112
TGCGGCT3900.033.460815145
CTACTAA11950.030.940758145
AGCGCAT1306.4813835E-427.884016145
CGGTCTG2507.823837E-826.09944145
CGACCTA1100.023.72676345-49
TCTACTA12000.023.561993145
CATGGGG42750.023.2005625-29
CGCGAAA1500.023.19950190-94
Produced by FastQC (version 0.11.5)