FastQCFastQC Report
Sun 19 Mar 2023
SRR4895708_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895708_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences832340
Sequences flagged as poor quality0
Sequence length151
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG155121.8636614844895114No Hit
CTGTAGCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC41810.5023187639666482No Hit
CTGTAGCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT16470.19787586803469737No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTAGC833300.0144.04591
TGTAGCC833900.0143.942262
GTAGCCA271650.0143.926973
TAGCCGT95750.0143.842424
GTAGCCG269900.0143.81823
TAGCCGA82100.0143.691994
GTAGCCT256500.0143.628623
TAGCCTA79750.0143.40524
TAGCCAT117050.0143.233284
TAGCCTG89350.0142.927694
TAGCCGG89300.0142.603974
TAGCCAG81700.0142.100714
AGCCGAT28350.0141.929785
AGCCATG59200.0141.904655
AGCCTAG25900.0141.713245
TAGCCAA73500.0141.668174
AGCCGTA28100.0141.139575
TAGCCTT87300.0140.997594
AGCCTAT28650.0140.69535
AGCCTGA26450.0140.40225
Produced by FastQC (version 0.11.5)