FastQCFastQC Report
Sun 19 Mar 2023
SRR4895708_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895708_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences832340
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG409304.917461614244179No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA65950.7923444746137397No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA56150.6746041281207199No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG27420.3294326837590408No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC12330.1481365788019319No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC10470.12578994161039958No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA10060.12086406997140592No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT325100.065.3352661
AGTGGTA341800.062.0607575
AGCAGTG342900.061.8939672
GGTATCA348200.061.0658078
CAGTGGT348350.060.9464344
GTGGTAT348300.060.7985046
GTATCAA349950.060.781159
TGGTATC348900.060.7770587
GCAGTGG353200.060.0479623
CGCCGAA907.481064E-540.278046145
CGGTCTG2404.3655746E-1133.22939145
CGACAGT950.004726690730.526518145
CGTGGGC4400.028.011549145
AAATATA19300.024.79291145
CTGACGA1550.001808165823.387253145
TACGCAC1750.023.19903850-54
GCCCCAA7250.023.000154145
CATGGGG51950.022.74661825-29
CACGGCT3355.4957127E-821.641933145
GAAACCG7250.020.7988985-89
Produced by FastQC (version 0.11.5)