FastQCFastQC Report
Sun 19 Mar 2023
SRR4895707_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895707_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1183838
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG134221.1337699921779838No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA115010.9715011682341672No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT38140.3221724594074527No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG28240.23854615242963986No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA20480.17299664312177848No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC19880.16792838209282013No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC13640.11521846739165326No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT236300.0101.866041
GGTATCA239500.0100.167768
GTGGTAT239200.099.966
GTATCAA240800.099.7733159
AGTGGTA241250.099.1105965
TGGTATC241650.099.0365457
AGCAGTG242850.098.794342
CAGTGGT243650.098.261664
GCAGTGG250000.095.56283
CGGTCTG3000.036.248363145
CCGCAAG11200.030.422733145
AGCGCAT1555.5029584E-528.063248145
CATGGGG75250.024.70396225-29
CGACGTA651.4897523E-924.53735485-89
CGACCTA4550.023.9000245-49
CCGCGAA1850.022.72870390-94
GAGGTAT2603.2050702E-622.3076296
ATGGGGC29000.021.75085425-29
TACGCAC5000.021.74901850-54
AAACCGC7100.021.6469185-89
Produced by FastQC (version 0.11.5)