FastQCFastQC Report
Sun 19 Mar 2023
SRR4895706_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895706_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences415273
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTGATCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG8820.21239040342136378No Hit
CGCTGATCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC4200.10113828734350655No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG351.2732926E-11144.179897
GCTGATC415700.0144.023822
CTGATCG137100.0143.916993
CGCTGAT416200.0143.850781
CTGATCA122250.0143.826083
TGATCGA39900.0143.457184
CTGATCT142900.0143.423193
TGATCAG48400.0142.988334
TGATCTG56050.0142.893724
GATCAGT16650.0142.880985
ATCGGTA4600.0142.61276
GATCTGG21150.0142.475655
TGATCGG40850.0142.238684
TGATCGT55500.0142.231524
TGATCAT40350.0142.035954
TGATCAA33150.0142.005234
GATCGTA16550.0141.566385
GATCGAT13200.0141.449225
CTGATCC14600.0141.217293
GATCAAT10650.0140.795385
Produced by FastQC (version 0.11.5)