FastQCFastQC Report
Sun 19 Mar 2023
SRR4895706_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895706_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences415273
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG142063.420882166671081No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA21660.5215845961572267No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10790.25982907629438945No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGTGCGATGGCTCA9120.21961456680304284No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG8510.20492543459362877No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC8270.19914610388828552No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT7340.17675119740508052No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGGCGCAGTGGCTCA5680.1367774933597898No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC5430.13075735720839063No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCCGGGCGCGGTGGCTCAT4990.12016191758192803No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT4870.11727225222925641No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA4240.10210150912773044No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT191000.089.958641
AGCAGTG196350.087.5285952
GGTATCA196250.087.499298
GTATCAA196550.087.34479
AGTGGTA195750.087.315295
GTGGTAT196150.087.1002666
TGGTATC196600.087.011567
CAGTGGT196950.086.930574
GCAGTGG204800.083.633923
GACTACT3050.052.29387145
CGCCGAA450.00963608348.33221145
TACTAGA2200.046.13529145
CCGCAAG3800.040.06486145
TGCGGCT2002.237357E-1036.249157145
CATGCGA850.002740460234.116856145
CGGTCTG850.002740460234.116856145
CGCAAGG3150.032.221474145
CGCTGTT2151.8055289E-830.348131145
AGGTATC1907.1301856E-626.716347
GAGGTAT2001.0617192E-525.3805226
Produced by FastQC (version 0.11.5)