FastQCFastQC Report
Sun 19 Mar 2023
SRR4895705_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895705_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences713031
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGATTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT105461.4790380782883212No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTGC714450.0143.911062
CGGATTG714550.0143.890911
GATTGCG272950.0143.754653
GATTGCA195700.0143.540543
ATTGCGT114000.0143.5084
GATTGCT222100.0143.424593
ATTGCGA70300.0142.870394
ATTGCGG85700.0142.60524
ATTGCAG71100.0142.479774
ATTGCTG83800.0141.62254
ATTGCAT67850.0140.696914
ATTGCTA61250.0140.67224
TTGCGTA28300.0140.382065
TTGCGAT26200.0139.525345
TTGCGGA23000.0139.501425
TTGCTGA23600.0139.315445
ATTGCTT78200.0138.948214
TTGCATG23350.0138.336765
TTGCTAG21750.0138.236665
TGCGTAA9350.0138.034586
Produced by FastQC (version 0.11.5)