FastQCFastQC Report
Sun 19 Mar 2023
SRR4895705_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895705_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences713031
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG289914.065882128547005No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA44710.6270414610304461No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24260.340237661476149No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAA12790.17937509028359216No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGCGATAGGAATCATGTC11280.15819789041430177No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTGTGGGGTTTTCTTCTA8590.1204716204484798No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT8380.11752644695672418No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC8240.11556299796222043No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA7580.10630673841670278No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT272850.067.0463941
AGTGGTA274600.066.5927055
GTATCAA277600.066.02059
GGTATCA278400.065.887498
GTGGTAT278100.065.756
TGGTATC280300.065.285687
CAGTGGT280200.065.266384
AGCAGTG283450.064.492462
GCAGTGG286500.063.7805863
CGGTCTG2404.3655746E-1133.22788145
GCTATCC4450.029.324707145
CGCGAAA1700.025.58724490-94
TACGCAC2050.025.4624350-54
TGCGGCT5850.024.785366145
CGACCTA2200.023.72635545-49
CGGAGCC3702.6011548E-1023.512606145
CGTGGGA5700.022.893852145
CGATAGG2900.022.49976235-39
CTACTAA12900.021.917757145
CATGGGG44450.021.85670525-29
Produced by FastQC (version 0.11.5)