FastQCFastQC Report
Sun 19 Mar 2023
SRR4895704_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895704_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517106
Sequences flagged as poor quality0
Sequence length151
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACACA519100.0143.678091
GACACAC519550.0143.553652
ACACACT182200.0141.93813
ACACACG160600.0141.674243
CACACGT50650.0141.528534
CACACTG61000.0140.568854
CACACTA54550.0140.532124
ACACGTA15450.0140.499595
ACACGAT17250.0139.216985
CACACTT66650.0139.04014
CACACAT57400.0138.328774
CACACGG58500.0138.316514
ACACTAG17550.0138.069985
CACACGA50950.0137.439684
ACACGTG15450.0137.232165
CACACAG53350.0137.20464
ACACTGT21850.0136.972615
ACACGTT16100.0136.618975
CACTTAG7500.0136.541346
ACACGGT19650.0136.526665
Produced by FastQC (version 0.11.5)