FastQCFastQC Report
Sun 19 Mar 2023
SRR4895704_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895704_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517106
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG129622.506642738626123No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA18940.3662691981914733No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16200.3132819963411757No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG7770.15025932787474908No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC7130.13788275517978907No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC6210.12009143193078402No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC6050.11699728875704402No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA138150.082.291315
AAGCAGT138400.082.247451
GGTATCA140800.081.1981058
GTGGTAT140150.081.057426
GTATCAA141600.080.4833769
AGCAGTG141550.080.468362
CAGTGGT142000.080.1112444
TGGTATC142450.079.748667
GCAGTGG145000.078.3037643
CGGTCTG1604.584217E-836.248234145
GCCCCAA5850.032.220657145
CTGACGA1100.00966964426.362354145
CGACACA2001.0637754E-525.3762171
TACGCAC3500.024.44166850-54
AGGGGTA1500.001492881824.1678285
CGACCTA3400.023.8811945-49
CATGGGG32700.023.01620325-29
CGGAGCC1902.1919019E-422.893623145
CGCGAAA1150.022.69454890-94
TCTCGCA3850.022.59848870-74
Produced by FastQC (version 0.11.5)