FastQCFastQC Report
Sun 19 Mar 2023
SRR4895703_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895703_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences385565
Sequences flagged as poor quality0
Sequence length151
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGA36950.0143.819814
CTCTATC387250.0143.577012
TCTATCG135750.0143.466463
CCTCTAT388350.0143.170331
CTATCAG39400.0143.112054
CTATCGG47100.0142.985384
CTATCTG45550.0142.78544
TCTATCA109050.0142.68933
CTATCGT49850.0142.6194
TATCGAG11900.0141.804785
TCTATCT129550.0141.315873
CTATCTA37800.0140.776524
TATCGGA11750.0140.546365
TATCGAA11300.0140.399725
CTATCAT38500.0140.277084
TATCGTG15600.0140.068365
CTATCCG4750.0139.656084
ATCGGTA4600.0139.525686
CTATCAA30800.0139.059724
TATCAGA10450.0138.708085
Produced by FastQC (version 0.11.5)