FastQCFastQC Report
Sun 19 Mar 2023
SRR4895703_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895703_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences385565
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG166804.326118812651563No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA24010.6227224981520625No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG19610.5086042560917096No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11020.285814324432975No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC8650.22434608950501214No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC5840.15146603037101397No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC4670.12112095236860192No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA4640.12034287344546316No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT4600.11930543488127812No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT168900.078.55571
AGTGGTA172100.076.916565
GGTATCA174050.076.5962758
AGCAGTG173850.076.36072
GTATCAA174800.076.143219
GTGGTAT173850.076.14236
CAGTGGT174750.075.6769944
TGGTATC175950.075.233537
GCAGTGG179300.073.7161563
CGGTCTG1901.2914825E-1038.159496145
TGCGGCT2500.037.701588145
AAATATA8850.036.04671145
CCGGACG1301.65168E-533.462948145
GGGGTAT1952.6022826E-729.7409846
GCCCCAA4350.028.334522145
CCGCAAG1804.688598E-628.19563145
CCAATCC1050.007701092427.620209145
AGGGGTA1855.8079477E-627.4300275
CACGGGA2456.414302E-826.633774145
CTAGGCG1409.980974E-425.893946145
Produced by FastQC (version 0.11.5)