FastQCFastQC Report
Sun 19 Mar 2023
SRR4895702_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895702_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1033643
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG704686.817440837890838No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA110771.0716465936498385No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG34250.33135231409684No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29780.28810720916215754No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC18510.17907536741408786No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA17520.16949759249566823No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC13480.13041253121242052No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA11810.11425608261266221No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT507350.061.9099771
AGCAGTG526100.059.6484072
AGTGGTA526150.059.512955
CAGTGGT533600.058.7013134
GCAGTGG541350.057.8475463
GTGGTAT553150.056.621156
GGTATCA558850.056.3679668
GTATCAA559900.056.2724889
TGGTATC558450.056.1357157
CGGTCTG3250.044.617527145
CACGGCT3306.184564E-1126.364902145
CGACCTA4100.023.69396845-49
GCCCCAA11500.023.32721145
AAATATA21900.023.174627145
TACGCAC4250.022.85771250-54
CCGCAAG9700.022.423758145
CATGGGG68850.022.40812725-29
GAAACCG8750.021.21039685-89
CGGAGCC5650.020.53196145
ATGGGGC22800.020.28727325-29
Produced by FastQC (version 0.11.5)