FastQCFastQC Report
Sun 19 Mar 2023
SRR4895701_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895701_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences877133
Sequences flagged as poor quality0
Sequence length151
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACAACATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG9450.107737367081161No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACAAC880650.0143.628622
CCGACAA880950.0143.579711
GACAACT293500.0143.497513
GACAACG310500.0142.79343
ACAACGT111200.0141.810784
GACAACA257250.0141.294923
ACAACTG103050.0140.291584
CAACGTA36400.0139.257695
CAACTGT34450.0139.186685
CAACTAG31800.0138.994815
CAACGTG36300.0137.853595
ACAACGA89300.0137.750384
ACAACTA90550.0137.521034
ACAACGG114450.0137.34284
CAACGAT32450.0137.321665
ACAACTT107600.0136.905644
CAACTTG37750.0136.76075
CAACATG31150.0136.108265
CAACGTT36500.0135.912935
CAACGGT39800.0135.876225
Produced by FastQC (version 0.11.5)