FastQCFastQC Report
Sun 19 Mar 2023
SRR4895701_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895701_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences877133
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG164791.8787344678629125No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27810.3170556802674167No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA23050.2627879694413504No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC13050.14878017358826995No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG12050.13737939400296192No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTTACCTCGTTGCACTGC9540.10876343724383873No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC9110.10386110202215627No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT224300.090.0489351
AGTGGTA226000.089.258535
GTGGTAT226200.089.014256
GGTATCA228650.088.536118
AGCAGTG229100.088.077382
CAGTGGT229400.087.998824
GTATCAA230850.087.682379
TGGTATC230900.087.359367
GCAGTGG234800.085.727943
CGCGTTA400.006052625454.370487145
CGGTCTG3800.041.9702145
AGCGCAT1604.591857E-836.246994145
GCCCCAA10200.031.271915145
CGACAAC2401.5788828E-930.2109932
GGTCTGA6650.026.163244145
TACGCAC4750.025.6402750-54
CGCGAAA2950.024.57451290-94
CATGGGG57050.023.17853725-29
CGACCTA5200.023.14310345-49
TCGCATC2202.22983E-523.066269145
Produced by FastQC (version 0.11.5)