FastQCFastQC Report
Sun 19 Mar 2023
SRR4895700_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895700_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences417921
Sequences flagged as poor quality0
Sequence length151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATCCTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC186374.4594552558976455No Hit
CCATCCTCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG87772.1001576853041604No Hit
CCATCCTCTGTGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC33230.7951263516310499No Hit
CCATCCTCTGCGTTGATACCACTGCTTCCCAGGTACTCTGCGTTGATACC17750.4247214186413222No Hit
CCATCCTCTGCGTTGATACCACTGCTTCCCATGTACGCTGCGTTGATACC11820.28282857286424945No Hit
CCATCCTCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCG10250.24526166428583393No Hit
CCATCCTCTGGGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC8620.20625907767257448No Hit
CCATCCTCTGCGTTGATACCACTGCTTCCCATGCACTCTGCGTTGATACC8200.19620933142866717No Hit
CCATCCTCTGAGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC6980.16701721138684106No Hit
CCATCCTCTGCGTTGATACCACGGCTTCCCATGTACTCTGCGTTGATACC6510.1557710667805638No Hit
CCATCCTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCA5180.12394687034152387No Hit
CCATCCTCTGCGTTGATACCACTGCTTCGCATGTACTCTGCGTTGATACC5160.1234683109965759No Hit
CCATCCTCGTGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG4960.11868271754709624No Hit
CCATCCTCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT4770.11413640377009053No Hit
CCATCCTCATGTACTCTGCGTTGATACCACTGCTCCCCATGTACTCTGCG4750.11365784442514254No Hit
CCATCCTCTGCGTTCATACCACTGCTTCCCATGTACTCTGCGTTGATACC4650.11126504770040271No Hit
CCATCCTCATGTACTCTGCGTGGATACCACTGCTTCCCATGTACTCTGCG4460.10671873392339702No Hit
CCATCCTCTGCGTTGATACCACTGCTTCCCATGGACTCTGCGTTGATACC4340.1038473778537092No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAGT550.0144.247318
CGATTAG500.0144.24738
ATCCTCG124700.0143.78463
CCATCCT419850.0143.577351
CATCCTC419950.0143.543152
ATCCTCA122900.0143.1913
ATCCTCT161700.0142.819993
TCCTCGA34750.0142.58694
CCTCATG34950.0142.390055
CTCGATA3300.0142.061756
CCTCGAT10550.0140.829135
TCCTCAG33500.0140.156714
TCCTCAT60550.0139.959184
CTCATGT28050.0139.619056
CTCATAG3100.0139.594166
TCCTCTG93150.0138.982254
TCCTCGT46250.0138.321494
CTCGATG3450.0137.97576
CCTCGTA14200.0137.644445
CCTCAGG11650.0137.437355
Produced by FastQC (version 0.11.5)