FastQCFastQC Report
Sun 19 Mar 2023
SRR4895699_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895699_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences374195
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCTTACATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG8100.21646467750771659No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGTA100.007207104144.201529
ACCTTAC375000.0143.874662
CCTTACG122100.0143.788183
CACCTTA375500.0143.683091
CTTACGA34500.0143.574554
CCTTACA113400.0142.612013
CTTACGT44700.0142.588534
CCTTACT130250.0142.430153
CTTACGG42100.0142.14644
CTTACTG43650.0142.05424
CTTACAG36250.0141.615844
CTTACAT44050.0141.418984
TTACGAG9950.0140.578375
CTTACTA39400.0140.541584
TTACGAT13950.0140.066715
TTACGTG13600.0139.430165
TTACGTA13000.0139.209935
TTACGGA11550.0139.207525
TTACGGT15750.0138.250375
ACGAGAT1200.0138.193137
Produced by FastQC (version 0.11.5)