FastQCFastQC Report
Sun 19 Mar 2023
SRR4895699_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895699_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences374195
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG138783.708761474632211No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA19240.5141704191664774No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16440.439343123237884No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG11420.30518847125162013No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT8520.2276887718970056No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC6700.17905102954341987No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA4160.11117198252248159No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTTCAACTTTAAATTTGCC3750.1002151284757947No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT188100.097.2404251
AGTGGTA191700.095.263045
GTATCAA193850.094.655259
AGCAGTG193350.094.6000752
GTGGTAT193000.094.583816
GGTATCA193950.094.531698
CAGTGGT193650.094.303774
TGGTATC194650.093.856547
GCAGTGG194850.093.5369643
TATCGGC451.1931524E-464.441536145
CACGGGA1753.76764E-628.99869145
GTGATAT2954.802132E-1027.0326795
AGGGGTA1358.075341E-426.850645
ATGGGAG27450.024.66737425-29
GCATATT2101.5522339E-524.165577145
CGTGGGA2401.5942514E-624.165575145
CATGGGG27200.023.72135525-29
ACCTTAC2151.8623428E-523.6035862
ATGGGAT28450.023.59647625-29
TGATATC3452.9522198E-923.1148996
Produced by FastQC (version 0.11.5)