FastQCFastQC Report
Sun 19 Mar 2023
SRR4895698_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895698_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences341998
Sequences flagged as poor quality0
Sequence length151
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGGAGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT4620.135088509289528No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCTG100.007207429144.197568
CAAGGAG343600.0143.50511
AAGGAGC343700.0143.463332
AGGAGCT120500.0143.35993
GGAGCTA33900.0142.921454
AGGAGCA93850.0142.661093
AGGAGCG119650.0142.51033
GGAGCAT35150.0142.351494
GAGCATA9400.0141.896515
GAGCTAT12300.0141.852875
GAGCTTA12000.0141.193445
GAGCTGA9350.0141.11315
GGAGCGT47000.0140.97614
GGAGCTT46600.0140.793754
GAGCGAT12450.0140.722925
GGAGCGA34150.0140.608434
GAGCTAG11900.0140.562325
GAGCTTG17450.0140.478975
GAGCGTA13450.0140.445195
GAGCTAA9350.0140.3425
Produced by FastQC (version 0.11.5)