FastQCFastQC Report
Sun 19 Mar 2023
SRR4895697_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895697_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences411717
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG279616.791315393826341No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA46511.1296594505449131No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG42421.0303193698584223No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC19790.48066997476421913No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT17140.4163053748084242No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC11980.29097656885676326No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9620.233655642103678No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG8490.20620960514139566No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA6910.1678337304507708No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACAGGATCCGGAGCTGGT6830.16589064818795435No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC6640.1612758278137653No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGTTTCAAAGTGGAGAACC5240.12727188821447744No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA4890.11877090331465547No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTAAACCTAGCCCCAAACCC4400.10686952445490469No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT246200.066.91091
AGCAGTG257650.063.86072
GTATCAA259950.063.475529
GGTATCA260250.063.326468
AGTGGTA259650.063.2214785
CAGTGGT259700.063.2169844
GTGGTAT260800.062.94276
TGGTATC262250.062.6223347
GCAGTGG266100.061.72383
AAATATA12350.046.96203145
CCGCAAG4700.038.562572145
GGTCTGA2353.45608E-1133.935062145
CGTGGGG1153.1594982E-431.52071145
CCACGTT1000.006064129529.0060983
CAGGGGG3503.6379788E-1226.927692145
GCGAATC950.025.94778385-89
CGACCTA950.025.94652245-49
CCGCGAA850.025.58739990-94
CGCAAGG3501.2914825E-1024.85633145
TACGCAC1000.024.64919550-54
Produced by FastQC (version 0.11.5)