FastQCFastQC Report
Sun 19 Mar 2023
SRR4895696_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895696_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences614875
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCAAGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC136682.222890831469811No Hit
CATCAAGTTGATACCACTGCGTCCCATGTACTCTGCGTTGATACCACTGC8480.1379142102053263No Hit
CATCAAGTTGGTACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC7330.11921122179304737No Hit
CATCAAGTTGATACCACTGCTGCCCATGTACTCTGCGTTGATACCACTGC6330.102947753608457No Hit
CATCAAGTTGATACCACTGCTTCGCATGTACTCTGCGTTGATACCACTGC6220.10115877210815206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAAGT615450.0144.085882
CATCAAG615550.0144.062451
TCAAGTG226800.0143.781333
CAAGTTG110750.0143.184664
TCAAGTT216550.0142.994343
CAAGTGG83650.0142.847154
CAAGTGT80950.0142.712054
TCAAGTA158500.0142.452093
CAAGTAG61300.0142.344244
CAAGTTA51400.0141.56084
CAAGTGA61550.0141.180274
CAAGTAT51000.0141.11574
TCAAGTC17950.0140.610763
CAAGTTT54900.0137.790154
CAAGTAA46600.0137.1084
CAAGTCG6000.0137.015174
AAGTGAG23250.0136.472375
AGTTGAT36800.0136.192126
AAGTTAG22850.0135.705445
AAGTTGA50850.0134.866655
Produced by FastQC (version 0.11.5)