FastQCFastQC Report
Sun 19 Mar 2023
SRR4895696_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895696_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences614875
Sequences flagged as poor quality0
Sequence length151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG13587122.097336857084773No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA185833.0222402927424272No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA36460.5929660500101647No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA23760.386420004065867No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCCACGCAG19830.3225045741004269No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCCTGACGTGGCCAAAAAA19010.3091685301890628No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG17940.2917666192315511No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13890.22589957308396016No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT13830.22492376499288472No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG13410.21809310835535678No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA13390.217767838991665No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT13270.21581622280951412No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAATCCTGACGTGGCCAAAAAA13080.21272616385444196No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC13060.21240089449075014No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT12380.2013417361252287No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG12380.2013417361252287No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG12090.1966253303516975No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC11580.1883309615775564No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG11310.183939825167717No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG11300.18377719048587113No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA11240.18280138239479568No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACTTGA11140.18117503557633666No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG11100.18052449684895305No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA10800.17564545639357593No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG10700.1740191095751169No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC10380.168814799756048No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA10360.16848953039235617No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG9460.15385240902622485No Hit
AGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT9340.151900792844074No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA9330.15173815816222808No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG9300.15125025411669038No Hit
TGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA9060.14734702175238867No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA8950.14555804025208374No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC7990.129945110794877No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC7960.1294572067493393No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG7880.12815612929457207No Hit
TACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT7750.12604187843057532No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC7630.12409026224842448No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAG6850.11140475706444398No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC6840.11124212238259809No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGGA9550.056.93408145
CATCGGA2109.094947E-1237.974125145
AAGCAGT931250.036.2753371
AGCAGTG934850.036.0920452
GGTATCA938750.035.9671488
AGTGGTA936450.035.9345325
GTATCAA942500.035.84429
GCAGTGG943300.035.66883
GTGGTAT944550.035.6081286
CAGTGGT945650.035.580164
TGGTATC948950.035.488867
CGTGGGG1458.6948785E-734.998096145
CGCTGTT1801.2916462E-732.22047145
CACGGGA3350.030.296858145
CATGCGA2354.2942702E-827.764446145
CGGAGCC2354.2942702E-827.764446145
CGCAAGG2954.820322E-1027.032427145
CAAGGGA6450.024.727337145
CATAGGA5750.023.955217145
GATGGGA4750.022.893492145
Produced by FastQC (version 0.11.5)