FastQCFastQC Report
Sun 19 Mar 2023
SRR4895695_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895695_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461679
Sequences flagged as poor quality0
Sequence length151
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCATTCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG129522.8054124185852074No Hit
ATCATTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC71661.5521607004000615No Hit
ATCATTCCCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGC29960.6489357324028167No Hit
ATCATTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT14800.32056905338990943No Hit
ATCATTCCTATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC13610.29479356869166673No Hit
ATCATTCCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCG12510.27096749039917345No Hit
ATCATTCCATGTACTCTGCGTGGATACCACTGCTTCCCATGTACTCTGCG9850.21335170107368973No Hit
ATCATTCCATGTACTCTGCGTTGATACCACTGCTCCCCATGTACTCTGCG9580.20750348185644135No Hit
ATCATTCCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCG8930.19342443559269537No Hit
ATCATTCCATGTACTCTGCGGTGATACCACTGCTTCCCATGTACTCTGCG8800.19060862633994616No Hit
ATCATTCCCATGTACTCTGCGTTGATACCACTGCTTCGCACGTACTCTGC6840.14815488683695813No Hit
ATCATTCCCATGTACTCTGCGTTGATACCACTGCTTCGCACGGACGCGGC5100.1104663629924688No Hit
ATCATTCCGTGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG4690.10158573381072131No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTAG351.2732926E-11144.19698
CATTCCG132050.0144.03313
ATCATTC462300.0143.994161
TCATTCC462700.0143.869672
TTCCGTA13850.0143.155785
CATTCCT139200.0142.798453
ATTCCGT49700.0142.746234
ATTCCGG45100.0142.598274
CATTCCA153700.0142.414383
ATTCCGA36000.0141.593354
ATTCCTG44050.0141.414444
ATTCCAG41200.0140.871984
ATTCCTA44250.0140.286484
ATTCCAT77550.0139.827294
ATTCCCA30000.0137.227394
TCCGAAT3850.0136.706166
TCCTAGA3650.0136.29576
TCCGGAT4500.0136.185976
TCCGTGA5900.0135.642856
TTCCGAT12500.0135.545095
Produced by FastQC (version 0.11.5)