FastQCFastQC Report
Sun 19 Mar 2023
SRR4895695_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895695_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461679
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG6401513.865694562672331No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA90431.9587202363546967No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28930.626625859092573No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA16320.35349236157590014No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTGGGAAGATTTATAGGTA14000.30324099644991437No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA11300.24475880427743085No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG9690.2098860896856907No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG9130.1977564498276941No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTGGAGGGCAAGTCTGGTG7310.15833512028920527No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT6790.14707188327820847No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA6690.14490587616070905No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAATGATAGATCGGAAGAGCA6240.1351588441319618No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC6180.13385923986146217No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG5970.12931062491471346No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG5970.12931062491471346No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT5970.12931062491471346No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT5900.1277944199324639No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG5720.12389560712096499No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC5520.11956359288596621No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCAAAATAGTGGGAAGATT5380.11653118292146707No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA5140.11133276583946855No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT5050.1093833594337191No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA4990.10808375516321947No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAATGATAGATCGGAAGAGCAC4960.10743395302796965No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA4950.10721735231621972No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG4920.10656755018096989No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC4900.10613434875747002No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG4800.10396834163997064No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACAATACAGGACTCTTTCG4790.10375174092822068No Hit
AGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT4770.10331853950472081No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT533350.069.584361
AGTGGTA555050.066.772475
AGCAGTG556200.066.706822
CAGTGGT562350.065.951514
GTGGTAT566750.065.381226
GGTATCA569750.065.329698
GCAGTGG567350.065.306373
GTATCAA570300.065.277979
TGGTATC570250.065.018097
CCGACCT553.2174916E-452.72625145
AGCGTTG450.00963661448.332397145
CATCGGC657.333165E-444.614517145
CGTGGGC4400.036.2493145
CACGGGC800.002035675636.249294145
CACGGGA3400.034.11698145
TGCGGGC1402.7438648E-531.070824145
CACGGGT1204.055157E-430.207747145
CGCGGGC1050.007704955527.61851145
GTCTACT1358.077005E-426.85133145
CGTGGGA3850.026.363125145
Produced by FastQC (version 0.11.5)