FastQCFastQC Report
Sun 19 Mar 2023
SRR4895693_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895693_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1159365
Sequences flagged as poor quality0
Sequence length151
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCTAA1160900.0144.024121
GGCTAAC1161100.0143.999312
GCTAACG377150.0143.9493
GCTAACT421550.0143.925873
CTAACTG146900.0143.480384
CTAACGT130000.0143.329184
CTAACGG134200.0143.24954
GCTAACA335250.0143.205753
CTAACGA110300.0142.909184
CTAACTA125200.0142.373664
CTAACTT148250.0141.78474
CTAACAT118100.0141.285934
TAACGAT40200.0141.167315
TAACGGT46000.0140.768025
TAACGTG41900.0140.602665
TAACGGA37100.0140.135075
TAACGTA40500.0140.121635
CTAACAG116950.0139.8394
TAACTGT51750.0139.479135
TAACTAG42250.0139.43795
Produced by FastQC (version 0.11.5)