FastQCFastQC Report
Sun 19 Mar 2023
SRR4895693_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895693_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1159365
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG226261.9515855662366899No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49370.4258365570808158No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA31910.27523687535849367No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCATGGTGGCTCA25690.22158681692133192No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC20290.17500959577009828No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT18950.1634515445955329No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT15780.13610899069749388No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCATGGTGGCTCAC13750.11859940570915976No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG12240.10557503460946294No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT11860.10229737830622798No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT297200.094.145131
AGTGGTA299750.092.9733665
GGTATCA302750.092.4352348
GTGGTAT301350.092.4075556
AGCAGTG304100.091.790432
GTATCAA305900.091.5031369
CAGTGGT306200.091.06624
TGGTATC306300.090.985217
GCAGTGG314250.088.548833
CGGTCTG4250.049.472305145
CGCTGTT6750.033.297455145
GAGGTAT3850.026.3634196
CGGAGCC4150.026.20573145
CCGCAAG13650.026.026546145
CGACCTA3100.025.72470545-49
TGCGGCT8900.025.253689145
AGCGCAT2151.8655672E-523.605474145
CATGGGG79000.023.36479225-29
TACGCAC3650.021.8483850-54
AGGTATC4751.2732926E-1121.3682447
Produced by FastQC (version 0.11.5)