FastQCFastQC Report
Sun 19 Mar 2023
SRR4895692_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895692_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448414
Sequences flagged as poor quality0
Sequence length151
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCATGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT20850.4649721016738996No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGATG351.2732926E-11144.227258
AGCCATG449000.0144.034521
GCCATGC449250.0143.954362
CCATGCG167650.0143.840123
CATGCGT60900.0143.635184
CATGCGA47250.0143.616764
CCATGCT136400.0143.592833
CCATGCA129050.0143.389043
CATGCAG44300.0142.924974
CATGCAT45400.0142.79774
CATGCTG49700.0142.776284
CATGCTA38800.0142.740374
CATGCAA38400.0142.53714
CATGCGG57800.0142.35584
ATGCGAT15050.0142.310615
CATGCTT47000.0142.07924
ATGCGTA16450.0142.035345
ATGCGTT23450.0141.767095
ATGCTGA13700.0141.595375
ATGCGGT18600.0141.125585
Produced by FastQC (version 0.11.5)