FastQCFastQC Report
Sun 19 Mar 2023
SRR4895692_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895692_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448414
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG202744.521268292247789No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG35220.7854348882951915No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA27310.6090354003220239No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC20390.45471372437078234No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14190.3164486389809417No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAT5910.13179784752483198No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT5630.12555361786206498No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA5200.11596426516567279No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAGAAATGCAAACTATCA4690.10459084685134719No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT217650.084.4084551
AGCAGTG224400.081.837122
GGTATCA225650.081.480178
GTATCAA225800.081.3939369
AGTGGTA226400.081.0181055
GTGGTAT226350.081.0039756
CAGTGGT226550.080.996474
TGGTATC227850.080.534357
GCAGTGG230950.079.359173
TTGGCGT554.0579525E-665.909096145
AAATATA14400.029.70486145
CGCAAGG1606.819876E-527.1875145
CCATGCG1358.075723E-426.8518523
AAACCGC6900.025.21739285-89
AGAGAGT5300.024.622643145
GAAACCG7600.023.08552785-89
GCGCAGG7700.022.97402645-49
ATGGGAG29400.022.73639525-29
CATGGGG29800.022.67449825-29
CGCAGAT7850.022.35031990-94
Produced by FastQC (version 0.11.5)