FastQCFastQC Report
Sun 19 Mar 2023
SRR4895691_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895691_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206217
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACCTCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG41362.0056542380114153No Hit
AGCACCTCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCG4900.23761377577988235No Hit
AGCACCTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC3420.1658447169728975No Hit
AGCACCTCATGTACTCTGCGTTGATACCACTGCTCCCCATGTACTCTGCG2740.13286974400752605No Hit
AGCACCTCATGTACTCTGCGTGGATACCACTGCTTCCCATGTACTCTGCG2320.112502848940679No Hit
AGCACCTCATGTACTCTGCGGTGATACCACTGCTTCCCATGTACTCTGCG2110.10231940140725546No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTTGA151.2549081E-4144.230858
CTCGCTA151.2549081E-4144.230856
TCGAGCT151.2549081E-4144.230857
CGATAGT151.2549081E-4144.230858
CGGTTAG500.0144.230838
CGGATAT202.1835986E-6144.230838
TCAGTCG100.0072003976144.230837
AGCACCT206550.0143.986451
GCACCTC206950.0143.708152
CACCTCG64900.0142.11963
CACCTCA68800.0141.71523
ACCTCTA18200.0141.457184
ACCTCGA18050.0141.434134
CTCATGT11650.0141.135776
CACCTCT65800.0141.05253
ACCTCAG18950.0140.805834
ACCTCAT32000.0140.625064
CCTCATG16450.0140.285325
ACCTCGT21650.0140.233674
ACCTCGG24400.0139.797524
Produced by FastQC (version 0.11.5)