FastQCFastQC Report
Sun 19 Mar 2023
SRR4895691_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895691_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206217
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2213110.73189892200934No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA28301.3723407866470756No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG16710.810311468016701No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCATG12070.5853057701353428No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAT9340.452920952200837No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC8310.4029735666797597No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5550.26913397052619326No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA5470.26525456194203195No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGGCGCGGTGGCTCA5270.25555604048162855No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA4060.19687998564618825No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCTGGGCGTGGTGGCTCAC3230.1566311215855143No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG2810.13626422651866724No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT2340.11347270108671934No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA2330.11298777501369917No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCTGGGCGCGGTGGCTCAC2300.11153299679463867No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG2190.10619880999141681No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC2110.10231940140725546No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCGC259.381905E-486.986465145
AAGCAGT166750.061.802971
AGTGGTA172850.059.370155
AGCAGTG173650.059.194532
CAGTGGT174550.058.806194
GGTATCA177000.058.2098668
GTATCAA178250.057.9503149
GTGGTAT177350.057.849696
GCAGTGG177550.057.771713
TGGTATC178700.057.4532367
AAATATA6750.046.178145
CTCTATT2450.041.422127145
CAGGGGG1301.6502008E-533.456337145
TCTATTA2054.0184386E-728.288282145
GCACCTC1050.00767303227.6348552
GTGATAT1606.7870555E-527.1964595
AAACCGC3750.025.51603185-89
GAAACCG3800.024.79877585-89
CGGCTAA2750.024.250772115-119
ATGGGGG7700.023.92114625-29
Produced by FastQC (version 0.11.5)