FastQCFastQC Report
Sun 19 Mar 2023
SRR4895690_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895690_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1950588
Sequences flagged as poor quality0
Sequence length151
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA36315918.61792444124541No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGTTGATA514882.639614311171811No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC490532.5147801585983305No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCAGGTACTCTGCGTTGATA300951.5428680992603256No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCACGGACGCGGCGCGGATA300781.5419965671889708No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGGACTCTGCGTTGATA279221.431465793904197No Hit
AGATGTACTCTGCGTTGATACCACTGCGTCCCATGTACTCTGCGTTGATA266711.3673312867709635No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCACGGACGCTGCGTTGATA264501.3560013698433497No Hit
AGATGTACTCTGCGTTGATACCACTGCTGCCCATGTACTCTGCGTTGATA225551.1563179923182139No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACGCTGCGTTGATA200101.0258445145771429No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCACGGACTCTGCGTTGATA167040.8563571599948323No Hit
AGATGTACTCTGCGTTGATACCACTGCATCCCATGTACTCTGCGTTGATA154090.7899669227945624No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCATGTACTCTGCGTTGATA150560.7718698156658402No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCACGGACGCTGCGCTGATA142810.7321382065305436No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCACGGACGCTGCGTTGATA129700.6649277038513515No Hit
AGATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTA124000.6357057461647463Illumina Single End PCR Primer 1 (100% over 21bp)
AGATGTACTCTGCGTTGATACCACTGCTTCCCACGTACGCTGCGTTGATA122280.626887892266332No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCACGTACTCTGCGTTGATA108580.5566526606336141No Hit
AGATGTACTCTGCGTTGATACCACTGCTTAGAACGGAAGAGCGGCGGGTA105450.5406062172021975No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCACGGACTCTGCGTTGATA98210.5034892042809656No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCACGTACGCTGCGTTGATA95770.49098015572740117No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCACGGACGCGGCGCTGATA90720.4650905265489175No Hit
AGATGTACTCTGCGTTGATACCACTGCTTAGAACGGAAGCGCGGCGGGTA89950.46114299893160426No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCACGGACGCTGCGCGGATA79470.4074156100621966No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGGACGCTGCGTTGATA74160.3801930494804644No Hit
AGATGTACTCTGCGTTGATACCACCGCTTCCCATGTACTCTGCGTTGATA72350.37091379625015636No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCACGGACGCGGCGTTGATA58820.30155009668879335No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCACGGACGCGGCGCGGGTA55340.2837093225222343No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGATGTACTCTGCGTTGATAC54670.2802744608292474No Hit
AGATGTACTCTGCGTTGATACCACTGCGTCCCAGGTACTCTGCGTTGATA50250.25761462697402016No Hit
AGATGTACTCTGCGTTGAGACCACTGCTTCCCATGTACTCTGCGTTGATA50170.25720449423455904No Hit
AGATGTACTCTGCGTTGATACCACTGCTGCCCAGGTACTCTGCGTTGATA50030.2564867619405021No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCATGTACGCTGCGTTGATA48840.2503860374410178No Hit
AGATGTACTCTGCGTTGATACCACTGCGTCCATGTACTCTGCGTTGATAC48640.24936070559236495No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCATGGACTCTGCGTTGATA46790.2398763859923264No Hit
AGATGTACTCTGCGTTGATACCACTGCGTCCCACGTACTCTGCGTTGATA45610.23382692808527483No Hit
AGATGTACTCTGCGTTGATAGCACTGCTTCCCATGTACTCTGCGTTGATA44020.22567553988848493No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCACGGACGCGGCGTGGATA43540.22321474345171816No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCAGGGACTCTGCGTTGATA41320.21183355993167188No Hit
AGATGTACTCTGCGTTGATACCACTGCTGCCATGTACTCTGCGTTGATAC40690.2086037646084155No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCATGGACGCTGCGTTGATA40600.20814236527652175No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCAAGGACCCCGCGGTGAAAC40550.20788603231435854No Hit
AGATGTACTCTGCGTTGATACCACTGCTACCCATGTACTCTGCGTTGATA40530.20778349912949326No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCACGGACGCTGCGTGGATA38960.19973464411756864No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAGA38930.19958084434027074No Hit
AGATGTACTCTGCGTTGATACCACTGCTGCCCACGTACTCTGCGTTGATA37330.1913781895510482No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCAGGAACCCGGCGTGGATA36470.18696926260184107No Hit
AGATGTACTCTGCCTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA35960.18435466638777642No Hit
AGATGTACTCTGCGTTGATACCACTGCTTAGAACGGAAGAGCGGCGTGTA33660.172563350128269No Hit
AGATGTACTCTGCGTCGATACCACTGCTTCCCATGTACTCTGCGTTGATA31640.16220749845687557No Hit
AGATGTACTCTGCGTTGATACCACTGCTTAGAACGGACGCGCGGCGGGTA31180.1598492352049741No Hit
AGATGTACTCTGCGTTGATACCACTGCGTCCCATGGACTCTGCGTTGATA29690.15221051293251062No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACACTGCGTTGATA29130.14933958375628273No Hit
AGATGTACTCTGCGTTGATACCACTGCGTCGCACGGACGCGGCGCGGATA28710.1471863868741118No Hit
AGATGTACTCTCCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA28240.1447768570297777No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCACGGACGCTGCGCTGATA27750.1422647940005783No Hit
AGATGTACTCTGCGTTGATACCACTGCTCCCCATGTACTCTGCGTTGATA27370.14031666348813795No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTCGATA27200.13944513141678302No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCAGGTACGCTGCGTTGATA27160.13924006504705247No Hit
AGATGTACTCTGCGTTGATACCACCGCTTCCCACGTACTCTGCGTTGATA27040.13862486593786078No Hit
AGATGTACTCTGCGTTGATACCACTGCTGCGCACGGACGCGGCGCGGATA26620.1364716690556899No Hit
AGATGTACTCTGCGTTGATACCACTGCATCCCACGTACTCTGCGTTGATA26110.13385707284162518No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCAGGTACTCTGCGTTGATAC25460.13052474433350353No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCAGGAACTCTGCGTTGATA25050.12842281404376527No Hit
AGATGTACTCTGCGTTGATACCACTGCGGCCCATGTACTCTGCGTTGATA25040.1283715474513326No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGCTGATA24600.12611581738429642No Hit
AGATGTACTCTGCGCTGATACCACTGCTTCCCATGTACTCTGCGTTGATA23970.12288602206104006No Hit
AGATGTACTCTGCGTTGATACCACTGCTTAGAACGGAAGAGCGTCGTGTA23670.12134802428806082Illumina Single End PCR Primer 1 (95% over 21bp)
AGATGTACTCCGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA23580.12088662495616706No Hit
AGATGTACTCTGCGTTGATACCACTGCTTAGATGGGCAGAGCGTCGAGAC22750.11663149778425787No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCACGTACGCTGCGCTGATA22050.11304283631397302No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCAAGGACCCCGCGGTGATAC21990.11273523675937717No Hit
AGATGTACTCTGCGTTGATACCACTGCTGCCCATGGACTCTGCGTTGATA21980.11268397016694454No Hit
AGATGTACTCTGCGTTGATACCACTGCAACCCATGTACTCTGCGTTGATA21390.10965924121341872No Hit
AGATGTACTCTGCGTTGATACCACTGCATCCATGTACTCTGCGTTGATAC20880.10704464499935404No Hit
AGATGTACTCTGCGTTGATACCACTGCGTCCCACGGACTCTGCGTTGATA20750.1063781792977297No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGATGGGCAGAGCGTCGAGAC20470.10494271470961576No Hit
AGATGTACTTTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA20240.10376358308366503No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGCTGATA19630.10063632094527393No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCATGGACTCTGCGTTGATAC19610.10053378776040867No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTATG100.007188868144.339268
CTAATTG100.007188868144.339268
CTAATGT100.007188868144.339268
CATAGGT151.2527691E-4144.339258
AGATGTA1951500.0144.261581
GATGTAC1985000.0141.826932
TCTGCCT35400.0139.661019
TGTACTT23100.0139.645754
TGTACGT14000.0138.14624
TACGTGT2300.0138.056556
CTTTGCG5450.0137.718198
CTCTGCC40450.0137.559428
GTACGTG5250.0137.458915
GTACGTT4750.0136.735435
TACGGTT1750.0136.084326
TGTACGA16350.0134.621464
TGTACGG15750.0134.251544
GTACGTA3500.0134.022435
GTACTTT13250.0133.983545
TACGTGA1250.0132.78536
Produced by FastQC (version 0.11.5)