FastQCFastQC Report
Sun 19 Mar 2023
SRR4895690_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895690_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1950588
Sequences flagged as poor quality0
Sequence length151
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG113482758.178713290556495No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA1584218.121704839771391No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA259901.332418737324335No Hit
AAGCAGTGGTATCAACGCAGAGTACATCTAGATCGGAAGAGCACACGTCT243971.2507510555791381Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA178480.9150061417377734No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG139750.7164506292461555No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA113120.579927693598033No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT105950.5431695468238296No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG101280.5192280481577862No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT100720.5163571189815583No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG100440.5149216543934444No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC100350.5144602550615507No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT98280.5038480704279941No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG94700.4854946303371086No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC87650.4493516826720968No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA85440.43802176574448315No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC84990.4357147690850144No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA82820.4245899185271313No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA80330.4118245370114037No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG79320.406646611175707No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG78300.4014174187475777No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG77700.3983414232016192No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA75120.3851146423539979No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG74630.38260257932479846No Hit
AGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT74210.38044938244262755No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG73940.37906518444694626No Hit
TGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA72100.3696321314393404No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA71530.36670993567067983No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG65040.3334379171818959No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC63330.32467132987591435No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAG61820.31693007441858556No Hit
TACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT60120.30821475370503665No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC53980.27673706595139513No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCG44800.22967433409823088No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCAGTGGTATCAACGCAG39990.20501510313813065No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA37290.19117312318131763No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCCACGCAG35920.18414960001804584No Hit
AGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAG35300.18097107128722212No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCCGTGGTATCAACGCAGA33730.1729222162752975No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGCAAGCAGTGGTATCAACGCAG29660.15205671315521269No Hit
AAGCAGTGGTATCAACGCAGAGTACATGTGAAGCAGTGGTATCAACGCAG27100.13893246549245664No Hit
AAGCAGTGGTATCAACGCAGAGTACATCTAGATCGGAAGAGCCCACGTCT25050.12842281404376527Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
AGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC24700.12662848330862284No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAT24220.12416768687185607No Hit
AAGCAGTGGTATCAACGCAGAGTACATGTAAGCAGTGGTATCAACGCAGA20080.10294331760474276No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGGG47700.084.200195145
TTCGGGG950.083.94422145
TTCGGGT5900.074.95482145
TCCGGTT2950.071.26852145
TTCGGCG2050.070.72905145
CTCGGCG2950.068.81098145
CTCGGCT20900.066.60037145
TACGGGA3950.066.07347145
TCTGGGT3000.065.24755145
TCCGGGG4700.064.784805145
CACGGCC7700.064.023575145
GGGCCCG2850.063.59411145
TCCGGGT6350.062.792923145
CTCGGCC2350.061.699814145
CCCGGGG15100.059.534195145
TTCCGCT3900.059.48495145
CCCGGCG4900.059.18146145
CCCGGCC7850.059.106064145
CTCGGTT502.0110924E-457.997826145
TCCCGTT753.764053E-757.99782145
Produced by FastQC (version 0.11.5)