FastQCFastQC Report
Sun 19 Mar 2023
SRR4895689_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895689_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences919362
Sequences flagged as poor quality0
Sequence length151
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGATTC920800.0143.996632
ACAGATT922150.0143.785831
AGATTCG311350.0143.783683
AGATTCA272700.0143.536213
GATTCAG94150.0143.074864
GATTCGG104150.0142.908224
GATTCGT119700.0142.838134
GATTCGA86250.0142.802414
GATTCAT101400.0142.090264
GATTCTG101500.0142.02134
AGATTCT315600.0141.687483
ATTCGTA36150.0141.431055
ATTCGAG26450.0140.952135
GATTCAA78150.0140.717354
ATTCGGA27650.0140.05095
GATTCTA95150.0139.979644
ATTCGAT31900.0139.702625
ATTCAGT35000.0138.042725
ATTCGTG36700.0137.936055
ATTCTAG32900.0137.867395
Produced by FastQC (version 0.11.5)