FastQCFastQC Report
Sun 19 Mar 2023
SRR4895689_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895689_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences919362
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG287933.1318457800083097No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA41960.4564034624010999No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41230.44846317337457936No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT14480.15750052753975038No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC14240.15489002155842857No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG14230.1547812504758735No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT281700.078.8918841
AGTGGTA284650.077.789845
GGTATCA289150.076.997118
GTATCAA290200.076.7684869
GTGGTAT288450.076.731576
AGCAGTG290750.076.3822252
CAGTGGT292200.075.854324
TGGTATC292850.075.6529547
GCAGTGG302500.073.247533
CGGTCTG2900.035.000275145
CGGAGCC3950.025.696405145
CGACCTA3300.024.60491445-49
GCCCCAA9200.024.42954145
TACGCAC3800.022.89367150-54
CATGGGG56800.022.8226125-29
GAGGTAT3757.765266E-921.2656786
TCTCGCA4250.021.15287870-74
ATGGGGC20200.020.53008825-29
CCGCAAG12000.019.937656145
AGGTATC4051.8824721E-819.6904417
Produced by FastQC (version 0.11.5)