FastQCFastQC Report
Sun 19 Mar 2023
SRR4895688_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895688_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379667
Sequences flagged as poor quality0
Sequence length151
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTCGA151.256464E-4144.20986
CGACCGT42350.0143.699024
ACGACCT124850.0143.574513
ACACGAC381850.0143.378951
CACGACC382100.0143.285142
CGACCTG41050.0142.980244
CGACCAT43750.0142.561694
ACGACCG119050.0142.453373
ACGACCA115100.0142.205143
CGACCTT43500.0142.054954
GACCGTA13100.0142.008125
GACCGTT13800.0141.59735
CGACCAG37300.0141.310134
CGACCGA34100.0141.249474
GACCTTG14300.0141.184425
GACCGTC4600.0141.07485
CGACCTA40300.0140.81034
GACCTAG13900.0140.059885
GACCGAT10200.0139.968345
GACCATG15100.0139.912165
Produced by FastQC (version 0.11.5)