FastQCFastQC Report
Sun 19 Mar 2023
SRR4895688_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895688_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379667
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG84272.219576628993302No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18650.4912199374715211No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA11900.313432560638665No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC5330.1403861805213516No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT89300.081.671011
AGTGGTA91050.079.851895
GGTATCA91500.079.617628
GTGGTAT91950.078.991486
CAGTGGT92150.078.9773564
GTATCAA92450.078.7994849
AGCAGTG92800.078.658512
TGGTATC93300.077.848527
GCAGTGG95750.075.9322743
CGGTCTG855.3364092E-542.64543145
CGATAGG304.3971657E-529.00347735-39
GGTTCCG255.153534E-429.0019530-34
TACGCAC1950.024.54011250-54
CGGCGGT2100.024.16956335-39
CGACCTA2200.023.0679145-49
CATGGGG24800.022.57125725-29
TCCGGCG2200.022.41177735-39
TCTCGCA2300.021.43509570-74
CGTATGC3055.3241274E-721.392626145
CACGGGA1700.00308998621.322716145
Produced by FastQC (version 0.11.5)