FastQCFastQC Report
Sun 19 Mar 2023
SRR4895687_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895687_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences360765
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGTTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT69371.922858370407329No Hit
AATGTTGCGTTGATACCACTGCTTCCCATGTACTCGGCGTTGATACCACT7970.2209194350893241No Hit
AATGTTGCGTTGATACCACTGCTTCCCAGGTACTCTGCGTTGATACCACT3830.10616329189361495No Hit
AATGTTGCGTTGATACCACTGCTGCCCATGTACTCTGCGTTGATACCACT3670.10172827186672766No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGA650.0144.219688
CGATAGT202.1866854E-6144.219688
ATGTTGC361300.0144.000142
AATGTTG362400.0143.563051
TGTTGCA98300.0143.33943
TGTTGCG137000.0142.851183
GTTGCGA31150.0142.367754
GTTGCAT35900.0142.211044
GTTGCAG35300.0142.17694
TGTTGCT118100.0141.349953
GTTGCGT60400.0140.996224
GTTGCTG42300.0140.639774
TTGCGAT12050.0140.629155
GTTGCTA32050.0140.394824
TGCGATA3200.0139.712816
GTTGCAA27350.0139.473884
GTTGCGG45950.0138.413244
TTGCAGT12500.0137.874015
TTGCGTA10900.0137.60415
TTGCTAG11100.0137.073675
Produced by FastQC (version 0.11.5)