FastQCFastQC Report
Sun 19 Mar 2023
SRR4895687_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895687_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences360765
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG305458.466730420079553No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA41421.148115809460452No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAA12110.3356755782850332No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11490.31848987568084486No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT7470.20705999750530124No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA7200.1995759012099289No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG6060.16797638351835684No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA5000.1385943758402284No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC4850.13443654456502155No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG4560.1263980707662883No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTAAACCTAGCCCCAAACCC3620.10034232810832537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT241250.052.337121
GTATCAA241800.052.1663789
AGTGGTA242750.051.8272745
GGTATCA243500.051.7724088
GTGGTAT242900.051.7212266
TGGTATC244750.051.389527
CAGTGGT247500.050.861914
AGCAGTG248850.050.6151282
GCAGTGG253600.049.60993
CTTGCGA450.00963243548.33555145
CGGTCTG1303.688292E-739.040253145
CGCTGTT1553.4584446E-837.42107145
CGGAGCC1255.1460083E-429.001331145
CCGCAAG4100.026.525606145
CGCAAGG2507.7981895E-826.101198145
GGTCTGA2259.076848E-725.77896145
CGCGAAA850.025.58870190-94
TACGCAC1200.025.37334850-54
CACGGGA2601.13999704E-725.097303145
CGACCTA1100.025.0431345-49
Produced by FastQC (version 0.11.5)