FastQCFastQC Report
Sun 19 Mar 2023
SRR4895686_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895686_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences606946
Sequences flagged as poor quality0
Sequence length151
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCCGTCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG15220.2507636593700263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCGT607550.0144.057361
ATCCGTC607550.0144.057362
TCCGTCA183950.0143.466873
TCCGTCG207100.0143.376043
CCGTCAG58650.0142.736334
CCGTCGA57350.0142.70294
TCCGTCT200500.0142.269653
CGTCATA18350.0142.246925
CCGTCAT70450.0142.164644
GTCGAAT6950.0142.136676
CGTCGAA17250.0141.703635
CCGTCAA54400.0141.693254
CGTCATG25650.0141.681635
CGTCAGT21600.0141.541065
CGTCTAA17850.0141.383975
CCGTCTA60500.0141.351264
CCGTCGT75850.0141.264684
CGTCGTA20850.0140.753345
CGTCGAT20950.0140.425665
CCGTCGG73300.0140.375184
Produced by FastQC (version 0.11.5)