FastQCFastQC Report
Sun 19 Mar 2023
SRR4895686_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895686_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences606946
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG180252.9697864389912776No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA27400.451440490587301No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23410.38570152863681445No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT14700.24219617560705567No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG13400.22077746619962893No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCACGGTGGCTCA8250.13592642508559247No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG6410.10561071330892698No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT214000.086.946371
GGTATCA219650.084.73598
AGTGGTA218100.084.706475
GTATCAA221450.084.262459
AGCAGTG220250.084.248652
TGGTATC221250.083.402177
GTGGTAT221350.083.331736
CAGTGGT222650.083.04744
GCAGTGG228300.080.9921343
CGGTCTG2700.056.393406145
AGGGGTA2109.094947E-1237.9792335
GGGGTAT2455.4569682E-1132.5536276
CGATAGG403.2604476E-728.99754335-39
AGGTATC2156.1136416E-726.9789077
ATCCGTC2705.5442797E-926.8562182
CAGGGGT3108.54925E-1025.7299884
GAGGTAT2301.1011507E-625.219416
CGACCTA2250.024.48681445-49
TACGCAC2300.023.9548950-54
AGAGGTA2852.772922E-722.8965725
Produced by FastQC (version 0.11.5)