FastQCFastQC Report
Sun 19 Mar 2023
SRR4895685_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895685_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1222575
Sequences flagged as poor quality0
Sequence length151
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCTG1224650.0143.953172
CAGATCT1224850.0143.929661
GATCTGA319450.0143.591053
GATCTGG448700.0143.268463
GATCTGT401200.0142.906523
ATCTGAG125500.0142.764194
ATCTGGA116350.0142.403354
ATCTGTG166500.0142.078584
ATCTGAT107900.0141.995334
ATCTGGT154600.0141.542164
ATCTGTA111750.0141.490634
GATCTGC62300.0141.307173
ATCTGGG176950.0141.022254
TCTGATG46000.0139.968485
ATCTGAA86650.0139.95684
TCTGAGT40550.0138.866275
TCTGGAT39500.0138.176865
ATCTGCG25400.0137.955544
CTGATGA10600.0137.398546
ATCTGTT126450.0136.845024
Produced by FastQC (version 0.11.5)