FastQCFastQC Report
Sun 19 Mar 2023
SRR4895685_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895685_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1222575
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG343742.811606649898779No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA49220.40259288796188375No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40690.3328221172525203No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT27310.2233809786720651No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG25120.20546796720037627No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC24900.20366848659591438No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC15320.12530928572889188No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT391250.081.9832461
AGTGGTA398300.079.8189245
GGTATCA399250.079.716548
GTATCAA399400.079.701499
GTGGTAT399400.079.595846
AGCAGTG403500.079.332572
CAGTGGT404150.078.7744144
TGGTATC404150.078.696217
GCAGTGG417400.076.378023
CGGAGCC5650.038.49791145
CGGTCTG4150.038.43607145
TACGCAC4750.025.64156350-54
CCGCAAG13150.025.362755145
AGCGCAT1801.5199298E-424.168133145
CATGGGG96100.023.4324425-29
CGCTGTT6750.022.556925145
CGACCTA5450.022.08119845-49
CTACTAA39350.021.557852145
ATGGGGG42750.021.16498425-29
TACCTCG3250.020.9689635-39
Produced by FastQC (version 0.11.5)