FastQCFastQC Report
Sun 19 Mar 2023
SRR4895684_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895684_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences674643
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACAC676800.0143.752142
GGACACT249100.0143.613023
AAGGACA678550.0143.381411
GACACTG79050.0143.126284
GGACACG209200.0142.91113
GACACGT73350.0142.74634
GACACTA75450.0142.309484
GACACTT94400.0142.158464
GACACAT73000.0141.948964
ACGTTAG2950.0141.776527
GGACACA199850.0141.767353
GACACGA63850.0140.832844
GACACAG67350.0140.366494
GACACGG71650.0140.195244
ACACGAT22400.0139.714055
ACACTTG32950.0139.187445
ACACGTA22800.0139.160555
ACACGTG22550.0137.825355
ACACTGA23750.0137.541245
ACACGGT25750.0137.499975
Produced by FastQC (version 0.11.5)