FastQCFastQC Report
Sun 19 Mar 2023
SRR4895684_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895684_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences674643
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG150272.227400269475856No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23960.3551507982740501No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA22830.338401198856284No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCAGTGGCTCAT10700.1586024015664581No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG8940.13251452990692855No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT7880.1168025162938028No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT7850.11635783666324265No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC7750.11487557122804208No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC7670.11368975887988166No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT186200.090.26531
GGTATCA191500.087.943378
AGTGGTA191350.087.639945
GTGGTAT191600.087.481256
GTATCAA192900.087.417869
CAGTGGT193000.086.8906864
AGCAGTG193350.086.733392
TGGTATC193900.086.480967
GCAGTGG198900.084.2403263
GCCCCAA5950.040.211647145
CGATAGG1450.028.00150335-39
TCCCGTA1050.0077052427.620121145
CGGTCTG2651.8189894E-1127.359554145
TGCGATA1550.026.1957330-34
TACGCAC3150.025.77686950-54
TGCGGCT6550.025.459003145
CGGTCCG2101.5536261E-524.167606145
CGACCTA3350.023.80512845-49
CATGGGG50300.023.4077725-29
AGGTATC2802.3410757E-723.302757
Produced by FastQC (version 0.11.5)