FastQCFastQC Report
Sun 19 Mar 2023
SRR4895683_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895683_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences578233
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGATC580100.0143.694272
GAGATCA177350.0143.394213
GAGATCG166400.0143.343383
AAGAGAT582350.0143.13911
AGATCAG54800.0142.5884
AGATCGT72200.0142.569054
AGATCTG70700.0142.43324
AGATCGA52800.0142.39174
GATCGGT14600.0142.191575
GAGATCT223850.0141.912353
GATCGAG18450.0141.431615
GATCAGT20300.0141.325745
GATCATG21300.0140.782295
AGATCAT68650.0140.596444
GATCGAT19100.0140.392475
GATCGTG21500.0140.143225
AGATCAA54750.0139.821724
GATCTAG21900.0139.55845
GATCTGT28150.0139.557245
AGATCTA69100.0139.367874
Produced by FastQC (version 0.11.5)